New Step by Step Map For high performance liquid chromatography
New Step by Step Map For high performance liquid chromatography
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크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
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are designed by reacting the silica particles with the organochlorosilane of the overall sort Si(CH3)2RCl, in which R is an alkyl or substituted alkyl group.
Separation Mechanism: Distinct column chemistries offer distinctive separation mechanisms depending on analyte Attributes like size, polarity, or charge. Comprehending the analytes and sought after separation system guides column range.
분석물의 피크 면적 값(=검출기의 응답)은 정량화를 위해 사용됩니다. 분석자는 분석을 수행하기 전, 분석물의 표준 용액(기지 농도의 시액)을 몇 가지 측정하고, 시료 농도와 획득한 피크 면적 값에 의해 도표된 검량선을 그립니다.
Inside a gasoline chromatograph the tension from the compressed fuel cylinder is ample to thrust the cellular section from the column. Pushing a liquid cell phase through a column, on the other hand, takes a terrific deal additional hard work, producing pressures in surplus of several hundred atmospheres.
Creating an optimized HPLC approach requires strategically modifying different parameters to accomplish the best possible separation for your particular analytes. Crucial parameters for optimization include:
The quick and economical putting together of the column may take yrs to master. Below are a few ideas and methods to put in place the right column
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
In case the cellular phase’s pH is adequately acidic, the solutes are present as neutral weak acids which can be extra soluble during the stationary period and consider extended to elute. Because the weak acid solutes do not need identical p
, such as, exhibits retention occasions for four weak acids in two cellular website phases with approximately equivalent values for (P^ primary ). Even though the order of elution is the same for both equally cell phases, Each individual solute’s retention time is affected in different ways by the choice of organic and natural solvent.
The selection of detector depends on the precise requirements of the Evaluation, taking into consideration elements like sensitivity, selectivity, and compatibility with the cell period.
In liquid–liquid chromatography the stationary phase is a liquid film coated on the packing material, ordinarily three–10 μm porous silica particles. As the stationary period could possibly be partly soluble while in the read more cellular phase, it could elute, or bleed from your column with time.